Hotspot

psbB

LSC · 75349-76875 · 1527 bp

Back to Gomphogyne

Pi 0.000655

MarkerSeek score 49.30

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 1
pi: 0.000655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CTCTCCAGGACTTCGAAACG	TGGAACTTTAGGTGGTTCGC	55.0	50.0	58.0	58.1	2323-2327 bp	1.0	79.5
psbB_p2	CTCTCCAGGACTTCGAAACG	ATCCATTTGAGGAACACGGG	55.0	50.0	58.0	57.9	2427-2431 bp	1.0	79.3
psbB_p3	AGTCCAACCAAGCAAACCAT	TGGAACTTTAGGTGGTTCGC	45.0	50.0	57.9	58.1	2547-2551 bp	1.0	78.9
psbB_p4	CTCTCCAGGACTTCGAAACG	ATCCCCAACGGTTTGTGTAG	55.0	50.0	58.0	57.8	2665-2669 bp	1.0	78.8
psbB_p5	CTCTCCAGGACTTCGAAACG	CTATCCCCAACGGTTTGTGT	55.0	50.0	58.0	57.8	2667-2671 bp	1.0	78.8