Hotspot

ndhC

LSC · 53423-53785 · 363 bp

Back to Gomphogyne

Pi 0

MarkerSeek score 24.30

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	AGGGAAACTCGATGGAATTCA	TTCGAATCGCCTAATTTGGT	42.9	40.0	57.0	55.4	477 bp	1.0	43.6
ndhC_p2	CGATCAAGTAAGGGAAACTCGA	TTCGAATCGCCTAATTTGGT	45.5	40.0	57.9	55.4	487 bp	1.0	42.7
ndhC_p3	TCGATCAAGTAAGGGAAACTCG	TTCGAATCGCCTAATTTGGT	45.5	40.0	57.9	55.4	488 bp	1.0	42.7
ndhC_p4	CAAGTAAGGGAAACTCGATGGA	TTCGAATCGCCTAATTTGGT	45.5	40.0	57.8	55.4	483 bp	1.0	42.0
ndhC_p5	TCAAGTAAGGGAAACTCGATGG	TTCGAATCGCCTAATTTGGT	45.5	40.0	57.8	55.4	484 bp	1.0	42.0