Hotspot

rpl32-trnL-UAG

SSC · 116336-117262 · 927 bp

Back to Gomphogyne

Pi 0.0504
MarkerSeek score 89.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
927
variable_sites
41
pi
0.0504
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAAAACAAAGGGCGTCGAG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1032-1059 bp 1.0 86.8
rpl32-trnL-UAG_p2 GAAAAACAAAGGGCGTCGAG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1033-1060 bp 1.0 86.8
rpl32-trnL-UAG_p3 TGAAAGCCTTTTCGCTAGCA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1006-1033 bp 1.0 86.3
rpl32-trnL-UAG_p4 TGAAAGCCTTTTCGCTAGCA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 1007-1034 bp 1.0 86.3
rpl32-trnL-UAG_p5 GAAAAACAAAGGGCGTCGAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1042-1069 bp 1.0 85.9