Hotspot

petA-psbJ

LSC · 65657-66644 · 988 bp

Back to Gomphogyne

Pi 0.0274

MarkerSeek score 74.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 27
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCGGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1132-1133 bp	1.0	78.8
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATTCGGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1129-1130 bp	1.0	77.6
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCGGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1127-1128 bp	1.0	76.3
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	ATTCGGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	1126-1127 bp	1.0	75.9
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	ATTCGGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1122-1123 bp	1.0	75.5