Hotspot

psaA-ycf3

LSC · 44849-45589 · 741 bp

Back to Gomphogyne

Pi 0.0096

MarkerSeek score 65.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 7
pi: 0.0096
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	915-919 bp	1.0	80.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	911-915 bp	1.0	78.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	913-917 bp	1.0	78.6
psaA-ycf3_p4	GTTCTCGAGAAATGACCCGG	ATCAAGCCGCTGAGTATTGG	55.0	50.0	58.4	58.0	939-943 bp	1.0	78.4
psaA-ycf3_p5	TGTTCTCGAGAAATGACCCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.6	58.0	940-944 bp	1.0	78.4