Hotspot

rpoB-trnC-GCA

LSC · 28328-29542 · 1215 bp

Back to Gomphogyne

Pi 0.0133

MarkerSeek score 67.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1215
variable_sites: 16
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCTCATTTCCACCCCCAAG	GCGTCTTTTGTTGATGAGGC	55.0	50.0	57.9	58.0	1321-1325 bp	1.0	80.1
rpoB-trnC-GCA_p2	CTCTCATTTCCACCCCCAAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1296-1300 bp	1.0	79.7
rpoB-trnC-GCA_p3	TCTCTCATTTCCACCCCCAA	GCGTCTTTTGTTGATGAGGC	50.0	50.0	58.2	58.0	1322-1326 bp	1.0	79.2
rpoB-trnC-GCA_p4	TTCTCTCATTTCCACCCCCA	GCGTCTTTTGTTGATGAGGC	50.0	50.0	58.2	58.0	1323-1327 bp	1.0	79.2
rpoB-trnC-GCA_p5	TCTCTCATTTCCACCCCCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1297-1301 bp	1.0	78.9