Hotspot

accD

LSC · 56859-58328 · 1470 bp

Back to Goldbachia

Pi 0.0636

MarkerSeek score 79.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1470
variable_sites: 91
pi: 0.0636
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAATAGAGGCCAGAAGCTCT	GGGTTTTCGTTTAATTCTGAT	45.0	33.3	55.9	52.4	1508-1523 bp	1.0	46.9
accD_p2	TGTAAATAGAGGCCAGAAGCTC	GGGTTTTCGTTTAATTCTGAT	45.5	33.3	57.6	52.4	1511-1526 bp	1.0	46.9
accD_p3	TGTAAATAGAGGCCAGAAGCT	GGGTTTTCGTTTAATTCTGAT	42.9	33.3	56.4	52.4	1511-1526 bp	1.0	46.9
accD_p4	GTAAATAGAGGCCAGAAGCTCT	GGGTTTTCGTTTAATTCTGAT	45.5	33.3	57.3	52.4	1510-1525 bp	1.0	46.9
accD_p5	AAATAGAGGCCAGAAGCTCT	GGGTTTTCGTTTAATTCTGATAAACT	45.0	30.8	55.9	56.7	1508-1523 bp	1.0	46.9