Hotspot

psbT-psbN

LSC · 73619-73683 · 65 bp

Back to Goldbachia

Pi 0.0769

MarkerSeek score 73.90

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 5
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GGGAACCGCCTAAAATTTCA	GATCCATTCGAGGAACACGG	45.0	55.0	56.3	58.4	124 bp	1.0	66.2
psbT-psbN_p2	GGGAACCGCCTAAAATTTCA	AGATCCATTCGAGGAACACG	45.0	50.0	56.3	57.4	125 bp	1.0	64.9
psbT-psbN_p3	GGGAACCGCCTAAAATTTCA	GGCAACCCTCTCAACAACTA	45.0	50.0	56.3	57.4	147 bp	1.0	64.6
psbT-psbN_p4	GGGAACCGCCTAAAATTTCAA	GATCCATTCGAGGAACACGG	42.9	55.0	57.1	58.4	124 bp	1.0	64.2
psbT-psbN_p5	GGGAACCGCCTAAAATTTCAA	GGCAACCCTCTCAACAACTA	42.9	50.0	57.1	57.4	147 bp	1.0	62.7