Hotspot

ycf4-cemA

LSC · 60055-60522 · 468 bp

Back to Goldbachia

Pi 0.0472

MarkerSeek score 78.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 22
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCTTACTTCTTGCGTGT	AGAGAGATCAACCAGGGCAA	45.0	50.0	58.0	58.3	581-587 bp	1.0	80.3
ycf4-cemA_p2	TTGGCTTACTTCTTGCGTGT	TCAACCAGGGCAAAAAGACT	45.0	45.0	58.0	57.5	574-580 bp	1.0	79.4
ycf4-cemA_p3	TTGGCTTACTTCTTGCGTGT	GAGAGATCAACCAGGGCAAA	45.0	50.0	58.0	57.5	580-586 bp	1.0	79.0
ycf4-cemA_p4	TTGGCTTACTTCTTGCGTGT	AGAGAGAGATCAACCAGGGC	45.0	55.0	58.0	58.5	583-589 bp	1.0	78.9
ycf4-cemA_p5	TTGGCTTACTTCTTGCGTGT	GCAGAGAGAGATCAACCAGG	45.0	55.0	58.0	57.4	585-591 bp	1.0	78.2