Hotspot

ndhC-trnV-GUA

LSC · 50099-50991 · 893 bp

Back to Goldbachia

Pi 0.0453
MarkerSeek score 76.40
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
893
variable_sites
39
pi
0.0453
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TGCCAAAACAGGAATAGCACT GTTCGAGTCCGTATAGCCCT 42.9 55.0 58.1 58.7 968-986 bp 1.0 71.4
ndhC-trnV-GUA_p2 TGCCAAAACAGGAATAGCACT TTCGAGTCCGTATAGCCCTA 42.9 50.0 58.1 57.1 967-985 bp 1.0 69.4
ndhC-trnV-GUA_p3 TGCCAAAACAGGAATAGCAC GTTCGAGTCCGTATAGCCCT 45.0 55.0 56.6 58.7 968-986 bp 1.0 69.3
ndhC-trnV-GUA_p4 TGCCAAAACAGGAATAGCACT AGGTCTACGGTTCGAGTCC 42.9 57.9 58.1 58.1 977-995 bp 1.0 67.8
ndhC-trnV-GUA_p5 TGCCAAAACAGGAATAGCAC TTCGAGTCCGTATAGCCCTA 45.0 50.0 56.6 57.1 967-985 bp 1.0 67.3