Hotspot

trnF-UUC-ndhJ

LSC · 47847-48421 · 575 bp

Back to Goldbachia

Pi 0.0524

MarkerSeek score 78.60

Primer available yes

Label trnF-UUC-ndhJ

Feature Metadata

label_name: trnF-UUC-ndhJ
feature_type: igs
region: LSC
length_bp: 575
variable_sites: 30
pi: 0.0524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-UUC-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAACGGAT	50.0	50.0	58.1	58.0	714-736 bp	1.0	83.1
trnF-UUC-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	687-709 bp	1.0	83.1
trnF-UUC-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	686-708 bp	1.0	83.1
trnF-UUC-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAACGGATCTT	50.0	50.0	58.1	57.9	711-733 bp	1.0	82.6
trnF-UUC-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	684-706 bp	1.0	81.5