Hotspot

trnS-AGC-trnG-GGA

LSC · 7901-8603 · 703 bp

Back to Goldbachia

Pi 0.0515
MarkerSeek score 82.20
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
703
variable_sites
35
pi
0.0515
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTT 50.0 45.0 57.8 56.5 802-811 bp 1.0 72.1
trnS-AGC-trnG-GGA_p2 CCAACGCTTTAGTCCACTCA ATCAAACCGAGAGACCCTTT 50.0 45.0 57.8 56.5 801-810 bp 1.0 72.1
trnS-AGC-trnG-GGA_p3 AACGCTTTAGTCCACTCAGC AATCAAACCGAGAGACCCTT 50.0 45.0 58.2 56.5 800-809 bp 1.0 71.8
trnS-AGC-trnG-GGA_p4 AACGCTTTAGTCCACTCAGC ATCAAACCGAGAGACCCTTT 50.0 45.0 58.2 56.5 799-808 bp 1.0 71.8
trnS-AGC-trnG-GGA_p5 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTTT 50.0 42.9 57.8 57.2 802-811 bp 1.0 70.1