Hotspot

psbK-psbI

LSC · 7178-7609 · 432 bp

Back to Goldbachia

Pi 0.064

MarkerSeek score 84.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 27
pi: 0.064
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	TTCTTCACGTCCCGGATTAC	50.0	50.0	57.9	57.3	563-570 bp	1.0	79.5
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	560-567 bp	1.0	77.5
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	561-568 bp	1.0	77.5
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	562-569 bp	1.0	77.5
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	CCGGATTACGTCCTGGATCA	50.0	55.0	57.9	59.0	552-559 bp	1.0	77.2