Hotspot

ycf4

LSC · 79316-79882 · 567 bp

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Pi 0.00235
MarkerSeek score 59.80
Primer available yes
Label ycf4

Feature Metadata

label_name
ycf4
feature_type
gene
region
LSC
length_bp
567
variable_sites
2
pi
0.00235
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4_p1 AAGTAACCCACGATTCCACG GCGATCACAATGGTGCTTTC 50.0 50.0 57.9 58.1 1196-1254 bp 1.0 82.6
ycf4_p2 AAGTAACCCACGATTCCACG GCTTTTCATGGAAAACGCGA 50.0 45.0 57.9 58.0 1380-1436 bp 1.0 82.5
ycf4_p3 AAGTAACCCACGATTCCACG TCGCTTTTCATGGAAAACGC 50.0 45.0 57.9 58.0 1382-1438 bp 1.0 82.5
ycf4_p4 TTTAGGGGGAAGCGTTTCTG GCTTTTCATGGAAAACGCGA 50.0 45.0 57.8 58.0 1034-1090 bp 1.0 82.3
ycf4_p5 TTTAGGGGGAAGCGTTTCTG TCGCTTTTCATGGAAAACGC 50.0 45.0 57.8 58.0 1036-1092 bp 1.0 82.3