Hotspot

ycf2

IRb · 10070-16627 · 6558 bp

Pi 0.000508

MarkerSeek score 52.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6558
variable_sites: 5
pi: 0.000508
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CTCCTTTTCGGTTTTTGTCGA	GGAAGGCCGGTTCATTGTAT	42.9	50.0	57.0	57.9	345 bp	1.0	64.2
ycf2_p2	CTCCTTTTCGGTTTTTGTCGA	TAAGGAAGGCCGGTTCATTG	42.9	50.0	57.0	57.9	348 bp	1.0	63.6
ycf2_p3	CTCCTTTTCGGTTTTTGTCGA	TCCAATTTAAGGAAGGCCGG	42.9	50.0	57.0	57.9	355 bp	1.0	63.5
ycf2_p4	TCGGTTTTTGTCGATTGAAACC	GGAAGGCCGGTTCATTGTAT	40.9	50.0	58.0	57.9	338 bp	1.0	63.5
ycf2_p5	CTCCTTTTCGGTTTTTGTCGA	CCAATTTAAGGAAGGCCGGT	42.9	50.0	57.0	58.2	354 bp	1.0	63.4