Hotspot

ycf1

SSC · 29085-36062 · 6978 bp

Pi 0.00325

MarkerSeek score 54.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6978
variable_sites: 34
pi: 0.00325
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAAACCCGAAAAGGAACCCG	TGCGGCTTCTCTCTGTTTTT	50.0	45.0	58.1	58.0	899-971 bp	1.0	79.8
ycf1_p2	TAAACCCGAAAAGGAACCCG	AAACTTGTATTTTGCGCCGG	50.0	45.0	58.1	57.9	514-586 bp	1.0	79.7
ycf1_p3	TAAACCCGAAAAGGAACCCG	GCCGGATAAACCCCAATTCT	50.0	50.0	58.1	57.9	499-571 bp	1.0	79.6
ycf1_p4	TAAACCCGAAAAGGAACCCG	TCCGCCTTTGTTAGAAGTCG	50.0	50.0	58.1	57.9	949-1021 bp	1.0	79.4
ycf1_p5	TAAACCCGAAAAGGAACCCG	GCTTGGCTTTCCTCTTCCTT	50.0	50.0	58.1	58.1	1054-1126 bp	1.0	79.4