Hotspot

psbB-ORF223

LSC · 69833-70406 · 574 bp

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Pi 0.00607
MarkerSeek score 57.80
Primer available yes
Label psbB-ORF223

Feature Metadata

label_name
psbB-ORF223
feature_type
igs
region
LSC
length_bp
574
variable_sites
5
pi
0.00607
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-ORF223_p1 CGATACCAAGGCAAACCCAT ACCTCTTCGTAACGAACACG 50.0 50.0 58.2 58.0 610-638 bp 1.0 82.0
psbB-ORF223_p2 CACGGCAATTAAACGACCAG ACCTCTTCGTAACGAACACG 50.0 50.0 57.8 58.0 656-684 bp 1.0 81.7
psbB-ORF223_p3 ATGCACGGCAATTAAACGAC ACCTCTTCGTAACGAACACG 45.0 50.0 57.7 58.0 659-687 bp 1.0 81.6
psbB-ORF223_p4 ACACGATACCAAGGCAAACC ACCTCTTCGTAACGAACACG 50.0 50.0 58.5 58.0 613-641 bp 1.0 80.1
psbB-ORF223_p5 CGATACCAAGGCAAACCCAT CCTCTTCGTAACGAACACGA 50.0 50.0 58.2 57.7 609-637 bp 1.0 79.8