Hotspot

trnH-GUG-trnI-CAU

IRa · 56737-57014 · 278 bp

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Pi 0.0216
MarkerSeek score 53.40
Primer available yes
Label trnH-GUG-trnI-CAU

Feature Metadata

label_name
trnH-GUG-trnI-CAU
feature_type
igs
region
IRa
length_bp
278
variable_sites
9
pi
0.0216
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-trnI-CAU_p1 TCACAATCCACTGCCTTGAG CCATTCAGCCATGGATGC 50.0 55.6 57.8 56.2 333-335 bp 1.0 50.7
trnH-GUG-trnI-CAU_p2 ACAAAATATTCTGGCCCGGG CCATTCAGCCATGGATGC 50.0 55.6 58.2 56.2 387-389 bp 1.0 50.0
trnH-GUG-trnI-CAU_p3 TTCACAATCCACTGCCTTGA CCATTCAGCCATGGATGC 45.0 55.6 57.3 56.2 334-336 bp 1.0 46.9
trnH-GUG-trnI-CAU_p4 ACAATCCACTGCCTTGAGC CCATTCAGCCATGGATGC 52.6 55.6 58.0 56.2 331-333 bp 1.0 43.8
trnH-GUG-trnI-CAU_p5 ATTCACAATCCACTGCCTTGA CCATTCAGCCATGGATGC 42.9 55.6 57.8 56.2 335-337 bp 1.0 42.9