Hotspot

ycf1-trnP-GGG

SSC · 36063-36342 · 280 bp

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Pi 0
MarkerSeek score 53.60
Primer available yes
Label ycf1-trnP-GGG

Feature Metadata

label_name
ycf1-trnP-GGG
feature_type
igs
region
SSC
length_bp
280
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-trnP-GGG_p1 GGCTAGAAGACCTTCTTTGCA TTGGTAGCGTGACATCATGG 47.6 50.0 57.9 58.0 433-436 bp 1.0 71.9
ycf1-trnP-GGG_p2 GGCTAGAAGACCTTCTTTGCA GTCAACTGGGGTATAACGCA 47.6 50.0 57.9 57.6 455-458 bp 1.0 68.9
ycf1-trnP-GGG_p3 GGCTAGAAGACCTTCTTTGCA TGTCAACTGGGGTATAACGC 47.6 50.0 57.9 57.6 456-459 bp 1.0 68.9
ycf1-trnP-GGG_p4 TCGGCTAGAAGACCTTCTTTG TTGGTAGCGTGACATCATGG 47.6 50.0 57.4 58.0 435-438 bp 1.0 67.6
ycf1-trnP-GGG_p5 GGCTAGAAGACCTTCTTTGCA CATCATGGGGTGGTGTAAGT 47.6 50.0 57.9 57.2 421-424 bp 1.0 65.6