Hotspot

ndhD-ccsA

SSC · 122270-122546 · 277 bp

Back to Gnaphalium

Pi 0.0642
MarkerSeek score 74.30
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
277
variable_sites
17
pi
0.0642
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 TCAGTTGACAAGGTTGAAGGT TGGTTCATTTACCTTAACCTCT 42.9 36.4 57.4 54.7 341-353 bp 1.0 46.7
ndhD-ccsA_p2 TCAGTTGACAAGGTTGAAGGT TGGTTCATTTACCTTAACCTCTAGT 42.9 36.0 57.4 57.4 341-353 bp 1.0 46.7
ndhD-ccsA_p3 TCAGTTGACAAGGTTGAAGGT ATGGTTCATTTACCTTAACCTCT 42.9 34.8 57.4 55.3 342-354 bp 1.0 46.7
ndhD-ccsA_p4 ATCAGTTGACAAGGTTGAAGGT TGGTTCATTTACCTTAACCTCT 40.9 36.4 57.8 54.7 342-354 bp 1.0 46.7
ndhD-ccsA_p5 CAGTTGACAAGGTTGAAGGT TGGTTCATTTACCTTAACCTCT 45.0 36.4 55.8 54.7 340-352 bp 1.0 46.7