Hotspot

ycf1-rps15

SSC · 113992-114395 · 404 bp

Back to Gnaphalium

Pi 0.0678

MarkerSeek score 78.40

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 404
variable_sites: 25
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATAATGGGAGCCGCTTGAG	TGTTTTCTTCTTGTTCTTGCA	50.0	33.3	58.0	54.4	478-504 bp	1.0	51.4
ycf1-rps15_p2	AATAATGGGAGCCGCTTGAG	TGTTTTCTTCTTGTTCTTGCAA	50.0	31.8	58.0	55.2	478-504 bp	1.0	49.8
ycf1-rps15_p3	AATAATGGGAGCCGCTTGAG	TTGTTTTCTTCTTGTTCTTGCA	50.0	31.8	58.0	55.2	479-505 bp	1.0	49.8
ycf1-rps15_p4	CAATAATGGGAGCCGCTTGA	TGTTTTCTTCTTGTTCTTGCA	50.0	33.3	58.3	54.4	479-505 bp	1.0	49.1
ycf1-rps15_p5	AATAATGGGAGCCGCTTGAG	CTTTGTTTTCTTCTTGTTCTTGCA	50.0	33.3	58.0	57.1	481-507 bp	1.0	48.2