Hotspot

petA-psbJ

LSC · 63546-64346 · 801 bp

Back to Gnaphalium

Pi 0.0395

MarkerSeek score 75.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 30
pi: 0.0395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	50.0	50.0	56.8	57.6	831-861 bp	1.0	74.6
petA-psbJ_p2	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	831-861 bp	1.0	73.4
petA-psbJ_p3	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	47.6	50.0	58.3	57.6	832-862 bp	1.0	73.4
petA-psbJ_p4	CGAGAAGGTTCAATTGTCCG	TGTGATCGGTTTAGTAGGCG	50.0	50.0	56.8	57.4	869-899 bp	1.0	72.6
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	GTGATCGGTTTAGTAGGCGT	50.0	50.0	56.8	57.4	868-898 bp	1.0	72.6