Hotspot

trnG-GCC-trnfM-CAU

LSC · 36626-36868 · 243 bp

Back to Gnaphalium

Pi 0.0894

MarkerSeek score 79.90

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 243
variable_sites: 21
pi: 0.0894
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GAACGAAACAAAAAGCGGGT	CTTGAGGTCACGGGTTCAAA	45.0	50.0	57.8	58.0	357-362 bp	1.0	84.6
trnG-GCC-trnfM-CAU_p2	GAACGAAACAAAAAGCGGGT	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	57.8	58.0	393-398 bp	1.0	84.1
trnG-GCC-trnfM-CAU_p3	GAACGAAACAAAAAGCGGGT	GTCACGGGTTCAAATCCTGT	45.0	50.0	57.8	58.1	351-356 bp	1.0	84.1
trnG-GCC-trnfM-CAU_p4	GAACGAAACAAAAAGCGGGT	TCACGGGTTCAAATCCTGTC	45.0	50.0	57.8	57.8	350-355 bp	1.0	83.6
trnG-GCC-trnfM-CAU_p5	GAACGAAACAAAAAGCGGGT	TGAGGTCACGGGTTCAAATC	45.0	50.0	57.8	57.8	355-360 bp	1.0	83.5