Hotspot

trnG-UCC-trnT-GGU

LSC · 31152-31536 · 385 bp

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Pi 0.1
MarkerSeek score 74.20
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
385
variable_sites
27
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.70
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 AATCAAACCGAAAGACCCCT ATTTGAACCGAGGGCTTACG 45.0 50.0 57.0 58.3 393-503 bp 1.0 78.9
trnG-UCC-trnT-GGU_p2 ATCAAACCGAAAGACCCCTT ATTTGAACCGAGGGCTTACG 45.0 50.0 57.0 58.3 392-502 bp 1.0 78.9
trnG-UCC-trnT-GGU_p3 ATCAAACCGAAAGACCCCTT AGGGCTTACGCTTTACACTC 45.0 50.0 57.0 57.6 382-492 bp 1.0 78.1
trnG-UCC-trnT-GGU_p4 ATCAAACCGAAAGACCCCTT GAGGGCTTACGCTTTACACT 45.0 50.0 57.0 57.6 383-493 bp 1.0 78.1
trnG-UCC-trnT-GGU_p5 ATCAAACCGAAAGACCCCTT GGGCTTACGCTTTACACTCT 45.0 50.0 57.0 57.6 381-491 bp 1.0 78.1