Hotspot

trnS-GCU-trnC-GCA

LSC · 8760-9478 · 719 bp

Back to Gnaphalium

Pi 0.0669

MarkerSeek score 82.20

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 46
pi: 0.0669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	787-806 bp	1.0	82.0
trnS-GCU-trnC-GCA_p2	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	769-788 bp	1.0	80.0
trnS-GCU-trnC-GCA_p3	TTTCGTCCACTCAGCCATCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.0	58.1	786-805 bp	1.0	78.9
trnS-GCU-trnC-GCA_p4	TCCACTCAGCCATCTCTCC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.1	58.1	781-800 bp	1.0	78.4
trnS-GCU-trnC-GCA_p5	ATTAGCAATCCGCCGCTTT	CCGGATTTGAACTGGGGAAA	47.4	50.0	58.2	58.1	802-821 bp	1.0	77.3