Hotspot

petA-psbJ

LSC · 64388-65490 · 1103 bp

Back to Gmelina

Pi 0.0149

MarkerSeek score 73.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 32
pi: 0.0149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	997-1266 bp	1.0	80.8
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.2	57.5	999-1268 bp	1.0	80.8
petA-psbJ_p3	CCCATTACGTGTCCAAGGTC	TCCGGATTAGGTTCATCCCT	55.0	50.0	58.3	57.5	1082-1351 bp	1.0	80.0
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	TCCGGATTAGGTTCATCCCT	55.0	50.0	58.3	57.5	1084-1353 bp	1.0	80.0
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.7	57.5	996-1265 bp	1.0	80.0