Hotspot

trnE-UUC-trnT-GGU

LSC · 31729-32318 · 590 bp

Back to Gmelina

Pi 0.00508

MarkerSeek score 62.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 6
pi: 0.00508
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CCATGATCAACCTCCCTTCG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	746-763 bp	1.0	80.4
trnE-UUC-trnT-GGU_p2	CCATGATCAACCTCCCTTCG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.0	57.9	711-728 bp	1.0	79.6
trnE-UUC-trnT-GGU_p3	AACCTCCCTTCGTAGTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	738-755 bp	1.0	79.6
trnE-UUC-trnT-GGU_p4	CCATGATCAACCTCCCTTCG	CCGATGACTTACGCCTTACC	55.0	55.0	58.0	58.2	743-760 bp	1.0	79.2
trnE-UUC-trnT-GGU_p5	AACCTCCCTTCGTAGTACCC	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.1	57.9	703-720 bp	1.0	78.8