Hotspot

rpl32-trnL-UAG

SSC · 115831-116305 · 475 bp

Back to Glyptopetalum

Pi 0.0254
MarkerSeek score 60.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
475
variable_sites
12
pi
0.0254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAATAAAAATCAA AGCGTGTCTACCGATTTCAC 28.6 50.0 52.9 58.0 544-562 bp 1.0 42.2
rpl32-trnL-UAG_p2 TTGTGCGACAAATAAAAATCA AGCGTGTCTACCGATTTCAC 28.6 50.0 52.9 58.0 545-563 bp 1.0 42.2
rpl32-trnL-UAG_p3 TGTGCGACAAATAAAAATCAA CAGCGTGTCTACCGATTTCA 28.6 50.0 52.9 58.0 545-563 bp 1.0 42.2
rpl32-trnL-UAG_p4 TTGTGCGACAAATAAAAATCA CAGCGTGTCTACCGATTTCA 28.6 50.0 52.9 58.0 546-564 bp 1.0 42.2
rpl32-trnL-UAG_p5 TGTGCGACAAATAAAAATCAA TCCTAAGAGCAGCGTGTCTA 28.6 50.0 52.9 57.9 554-572 bp 1.0 42.1