Hotspot

infA-rps8

LSC · 81503-81644 · 142 bp

Back to Glyptopetalum

Pi 0.0563

MarkerSeek score 82.00

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 8
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AACGAACCCGAAACATACCA	GGCGGAATGGGGATTGTAAT	45.0	50.0	57.5	58.0	320-325 bp	1.0	77.6
infA-rps8_p2	AACGAACCCGAAACATACCA	TTTAGGCGGAATGGGGATTG	45.0	50.0	57.5	57.9	324-329 bp	1.0	77.1
infA-rps8_p3	AACGAACCCGAAACATACCA	TAGGCGGAATGGGGATTGTA	45.0	50.0	57.5	58.2	322-327 bp	1.0	76.1
infA-rps8_p4	ACGAACCCGAAACATACCAT	GGCGGAATGGGGATTGTAAT	45.0	50.0	57.2	58.0	319-324 bp	1.0	75.8
infA-rps8_p5	ACGAACCCGAAACATACCAT	TTTAGGCGGAATGGGGATTG	45.0	50.0	57.2	57.9	323-328 bp	1.0	75.2