Hotspot

petD-rpoA

LSC · 79186-79407 · 222 bp

Back to Glyptopetalum

Pi 0.0811

MarkerSeek score 85.10

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 18
pi: 0.0811
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	ATTGGTACTGTAGTGGCCCT	TCACATTGAAGACGTGAAACA	50.0	38.1	58.0	56.1	364-371 bp	1.0	60.3
petD-rpoA_p2	GGTTGGGTATTGGAGCAACA	TCACATTGAAGACGTGAAACA	50.0	38.1	58.1	56.1	342-349 bp	1.0	60.2
petD-rpoA_p3	TGGTTGGGTATTGGAGCAAC	TCACATTGAAGACGTGAAACA	50.0	38.1	58.1	56.1	343-350 bp	1.0	60.2
petD-rpoA_p4	GGTACTGTAGTGGCCCTTTG	TCACATTGAAGACGTGAAACA	55.0	38.1	57.9	56.1	361-368 bp	1.0	59.8
petD-rpoA_p5	GTACTGTAGTGGCCCTTTGG	TCACATTGAAGACGTGAAACA	55.0	38.1	57.9	56.1	360-367 bp	1.0	59.8