Hotspot

rpoB-trnC-GCA

LSC · 27292-28296 · 1005 bp

Back to Glyptopetalum

Pi 0.0143

MarkerSeek score 69.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 14
pi: 0.0143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCCCTCATTTCCATCCCC	GCTTTTGTTGATCAGGCGAC	55.0	50.0	57.9	58.0	1088-1115 bp	1.0	82.1
rpoB-trnC-GCA_p2	GTTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1066-1093 bp	1.0	81.8
rpoB-trnC-GCA_p3	GTTCCCTCATTTCCATCCCC	AGCTTTTGTTGATCAGGCGA	55.0	45.0	57.9	58.1	1089-1116 bp	1.0	81.6
rpoB-trnC-GCA_p4	CGTTCCCTCATTTCCATCCC	GCTTTTGTTGATCAGGCGAC	55.0	50.0	58.3	58.0	1089-1116 bp	1.0	80.2
rpoB-trnC-GCA_p5	CGTTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.3	58.1	1067-1094 bp	1.0	79.8