Hotspot

atpF-atpH

LSC · 13144-13473 · 330 bp

Back to Glyptopetalum

Pi 0.0404

MarkerSeek score 78.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 330
variable_sites: 13
pi: 0.0404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCACGAAAAGGAA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.0	57.3	483-490 bp	1.0	77.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.9	57.3	511-518 bp	1.0	76.6
atpF-atpH_p3	CAGTGACCCACGAAAAGGAA	GAGGCAGAGGGAAAAATACGA	50.0	47.6	58.0	57.7	481-488 bp	1.0	73.1
atpF-atpH_p4	CAGTGACCCACGAAAAGGAA	AGGCAGAGGGAAAAATACGAG	50.0	47.6	58.0	57.7	480-487 bp	1.0	73.0
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GAGGCAGAGGGAAAAATACGA	50.0	47.6	57.9	57.7	509-516 bp	1.0	72.2