Hotspot

trnS-GCU-trnG-UCC

LSC · 7827-8712 · 886 bp

Back to Glyptopetalum

Pi 0.0235
MarkerSeek score 74.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
886
variable_sites
20
pi
0.0235
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TAATCCAACCGAGGGATCCC 50.0 55.0 58.0 58.6 979-999 bp 1.0 80.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TAATCCAACCGAGGGATCCC 50.0 55.0 58.0 58.6 978-998 bp 1.0 80.0
trnS-GCU-trnG-UCC_p3 AGCAATCCGACGCTTTAGTC TAATCCAACCGAGGGATCCC 50.0 55.0 58.4 58.6 981-1001 bp 1.0 77.2
trnS-GCU-trnG-UCC_p4 CGACGCTTTAGTCCACTCAG TAATCCAACCGAGGGATCCC 55.0 55.0 58.4 58.6 974-994 bp 1.0 77.0
trnS-GCU-trnG-UCC_p5 GCTTTAGTCCACTCAGCCAT TAATCCAACCGAGGGATCCC 50.0 55.0 57.6 58.6 970-990 bp 1.0 76.8