Hotspot

ndhA

SSC · 124555-126771 · 2217 bp

Back to Glycosmis

Pi 0.00427

MarkerSeek score 61.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 14
pi: 0.00427
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCGGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.0	57.9	2325-2341 bp	1.0	80.2
ndhA_p2	ACCGGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	50.0	50.0	58.0	57.8	2323-2339 bp	1.0	79.7
ndhA_p3	ACCGGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAAT	50.0	50.0	58.0	58.5	2423-2439 bp	1.0	77.0
ndhA_p4	ACCGGTTACCATAGGGAACA	TGGGGGAAGTTGATCGTTGA	50.0	50.0	58.0	58.9	2324-2340 bp	1.0	73.7
ndhA_p5	ACCGGTTACCATAGGGAACA	GCCCACCGGGTTTTATCAA	50.0	52.6	58.0	58.0	2423-2439 bp	1.0	72.9