Hotspot

rpl32-trnL-TAG

SSC · 118135-118872 · 738 bp

Back to Glycosmis

Pi 0.0257

MarkerSeek score 66.50

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 738
variable_sites: 26
pi: 0.0257
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	AGACAGAGATAAGGGCGGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	1289-1346 bp	1.0	85.2
rpl32-trnL-TAG_p2	AGACAGAGATAAGGGCGGAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	1290-1347 bp	1.0	85.2
rpl32-trnL-TAG_p3	CGAAATCCCTTTCTACCGGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	823 bp	0.333333	52.9
rpl32-trnL-TAG_p4	CGAAATCCCTTTCTACCGGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	824 bp	0.333333	52.9
rpl32-trnL-TAG_p5	CGAAATCCCTTTCTACCGGG	GCTGCATAGCCCAATACCAT	55.0	50.0	58.1	58.1	1425 bp	0.333333	52.3