Hotspot

psbJ-psbL

LSC · 67706-67847 · 142 bp

Back to Glycosmis

Pi 0.0141

MarkerSeek score 61.50

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 3
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCCTTCCAGTAGTATCGGCC	TGAACCGTACCAGTCTCTACT	55.0	47.6	58.3	57.6	244 bp	1.0	67.2
psbJ-psbL_p2	TCCTTCCAGTAGTATCGGCC	TGACACAATCAAACCCAAACG	55.0	42.9	58.3	57.6	280 bp	1.0	67.0
psbJ-psbL_p3	TCCTTCCAGTAGTATCGGCC	GACACAATCAAACCCAAACGA	55.0	42.9	58.3	57.3	279 bp	1.0	64.9
psbJ-psbL_p4	CCTTCCAGTAGTATCGGCCA	TGAACCGTACCAGTCTCTACT	55.0	47.6	58.6	57.6	243 bp	1.0	64.8
psbJ-psbL_p5	CCTTCCAGTAGTATCGGCCA	TGACACAATCAAACCCAAACG	55.0	42.9	58.6	57.6	279 bp	1.0	64.7