Hotspot

ycf4-cemA

LSC · 63808-64575 · 768 bp

Back to Glycosmis

Pi 0.00888

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 10
pi: 0.00888
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	ATGAGAGAGGGATCCACCAG	50.0	55.0	58.0	57.9	899-927 bp	1.0	82.9
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	ATGAGAGAGGGATCCACCAG	50.0	55.0	58.0	57.9	907-935 bp	1.0	82.9
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	ATGAGAGAGGGATCCACCAG	45.0	55.0	58.1	57.9	864-892 bp	1.0	82.4
ycf4-cemA_p4	TGACTCCGCGAGAAATTGAG	TAAGGGGAATGGATGCGTTC	50.0	50.0	58.0	57.7	842-870 bp	1.0	81.3
ycf4-cemA_p5	TGAGAATTTGACTCCGCGAG	TAAGGGGAATGGATGCGTTC	50.0	50.0	58.0	57.7	850-878 bp	1.0	81.2