Hotspot

accD-psaI

LSC · 61921-62704 · 784 bp

Back to Glycosmis

Pi 0.00937

MarkerSeek score 53.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 11
pi: 0.00937
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGTTCCATGGCTGTTTTCCT	AGAGGGAAAGCTGTTGAGAA	45.0	45.0	57.6	56.4	838-847 bp	1.0	66.0
accD-psaI_p2	AAAAGGTGTTCTGAGCGAGT	AGAGGGAAAGCTGTTGAGAA	45.0	45.0	57.4	56.4	864-873 bp	1.0	64.0
accD-psaI_p3	AAAGGTGTTCTGAGCGAGTT	AGAGGGAAAGCTGTTGAGAA	45.0	45.0	57.4	56.4	863-872 bp	1.0	64.0
accD-psaI_p4	CTGTTCCATGGCTGTTTTCC	AGAGGGAAAGCTGTTGAGAA	50.0	45.0	57.3	56.4	839-848 bp	1.0	63.2
accD-psaI_p5	TGTTCCATGGCTGTTTTCCT	AGAGGGAAAGCTGTTGAGAAT	45.0	42.9	57.6	56.9	838-847 bp	1.0	62.4