Hotspot

trnD-GTC-trnY-GTA

LSC · 33130-33602 · 473 bp

Back to Glycosmis

Pi 0.0141

MarkerSeek score 61.40

Primer available yes

Label trnD-GTC-trnY-GTA

Feature Metadata

label_name: trnD-GTC-trnY-GTA
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 9
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-trnY-GTA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	539-546 bp	1.0	76.2
trnD-GTC-trnY-GTA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	538-545 bp	1.0	76.2
trnD-GTC-trnY-GTA_p3	CGGTGCTCTGACCAATTGAA	TCTTCTTCTTCCTGGGTCGA	50.0	50.0	58.5	57.7	596-603 bp	1.0	75.5
trnD-GTC-trnY-GTA_p4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	560-567 bp	1.0	75.2
trnD-GTC-trnY-GTA_p5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	559-566 bp	1.0	75.0