Hotspot

rpoB-trnC-GCA

LSC · 28256-29482 · 1227 bp

Back to Glycosmis

Pi 0.00601
MarkerSeek score 63.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1227
variable_sites
11
pi
0.00601
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTCCCGCATTTCCATCTCC ACCAGCCAAGCAGAATAAGG 50.0 50.0 58.0 57.9 1371-1413 bp 1.0 79.8
rpoB-trnC-GCA_p2 ATTCCCGCATTTCCATCTCC TCCTTTGTTGATCAGGCGAC 50.0 50.0 58.0 58.2 1337-1379 bp 1.0 79.4
rpoB-trnC-GCA_p3 ATTCCCGCATTTCCATCTCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1297-1339 bp 1.0 78.1
rpoB-trnC-GCA_p4 ATTCCCGCATTTCCATCTCC CAGCCAAGCAGAATAAGGGA 50.0 50.0 58.0 57.6 1369-1411 bp 1.0 77.5
rpoB-trnC-GCA_p5 ATTCCCGCATTTCCATCTCC GCGACACCCAGATTTGAACT 50.0 50.0 58.0 58.5 1322-1364 bp 1.0 77.1