Hotspot

trnR-TCT-atpA

LSC · 10796-11053 · 258 bp

Back to Glycosmis

Pi 0.031

MarkerSeek score 70.30

Primer available yes

Label trnR-TCT-atpA

Feature Metadata

label_name: trnR-TCT-atpA
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 11
pi: 0.031
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-TCT-atpA_p1	ATGAAAGGCGTCCATTGTCT	TCAGGAACAAAAGGAGCGTT	45.0	45.0	57.5	57.7	372-397 bp	1.0	77.3
trnR-TCT-atpA_p2	ATGAAAGGCGTCCATTGTCT	TTCAGGAACAAAAGGAGCGT	45.0	45.0	57.5	57.7	373-398 bp	1.0	77.2
trnR-TCT-atpA_p3	AATGGGAATGAAAGGCGTCC	TCAGGAACAAAAGGAGCGTT	50.0	45.0	58.5	57.7	379-404 bp	1.0	77.0
trnR-TCT-atpA_p4	GGGAATGAAAGGCGTCCATT	TCAGGAACAAAAGGAGCGTT	50.0	45.0	58.5	57.7	376-401 bp	1.0	77.0
trnR-TCT-atpA_p5	GGGAATGAAAGGCGTCCATT	TTCAGGAACAAAAGGAGCGT	50.0	45.0	58.5	57.7	377-402 bp	1.0	77.0