Hotspot

trnS-GCT-trnR-TCT

LSC · 8859-10723 · 1865 bp

Back to Glycosmis

Pi 0.0274

MarkerSeek score 69.00

Primer available yes

Label trnS-GCT-trnR-TCT

Feature Metadata

label_name: trnS-GCT-trnR-TCT
feature_type: igs
region: LSC
length_bp: 1865
variable_sites: 71
pi: 0.0274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnR-TCT_p1	CCGACGCTTTAATCCACTCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1932-1957 bp	1.0	68.9
trnS-GCT-trnR-TCT_p2	CCGACGCTTTAATCCACTCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1938-1963 bp	1.0	68.2
trnS-GCT-trnR-TCT_p3	ACGCTTTAATCCACTCAGCC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.3	56.9	1929-1954 bp	1.0	66.9
trnS-GCT-trnR-TCT_p4	CGCTTTAATCCACTCAGCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.3	56.9	1928-1953 bp	1.0	66.8
trnS-GCT-trnR-TCT_p5	TCCGACGCTTTAATCCACTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.7	56.9	1933-1958 bp	1.0	66.6