Hotspot

psbI-trnS-GCT

LSC · 8613-8770 · 158 bp

Back to Glycosmis

Pi 0.0225

MarkerSeek score 63.00

Primer available yes

Label psbI-trnS-GCT

Feature Metadata

label_name: psbI-trnS-GCT
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 5
pi: 0.0225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCT_p1	CTGTCTAATGATCCAGGGCG	TGAGTGGATTAAAGCGTCGG	55.0	50.0	57.8	58.0	266-277 bp	1.0	83.7
psbI-trnS-GCT_p2	CTGTCTAATGATCCAGGGCG	TAGGAGAGATGGCTGAGTGG	55.0	55.0	57.8	57.9	279-290 bp	1.0	82.9
psbI-trnS-GCT_p3	CTGTCTAATGATCCAGGGCG	GGCTGAGTGGATTAAAGCGT	55.0	50.0	57.8	58.3	269-280 bp	1.0	81.6
psbI-trnS-GCT_p4	CTGTCTAATGATCCAGGGCG	TGGCTGAGTGGATTAAAGCG	55.0	50.0	57.8	58.3	270-281 bp	1.0	81.6
psbI-trnS-GCT_p5	CTGTCTAATGATCCAGGGCG	GAGTGGATTAAAGCGTCGGA	55.0	50.0	57.8	57.7	265-276 bp	1.0	81.5