Hotspot

trnH-GTG-psbA

LSC · 75-523 · 449 bp

Back to Glycosmis

Pi 0.0148

MarkerSeek score 65.00

Primer available yes

Label trnH-GTG-psbA

Feature Metadata

label_name: trnH-GTG-psbA
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 10
pi: 0.0148
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	518-566 bp	1.0	81.9
trnH-GTG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	532-580 bp	1.0	81.8
trnH-GTG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	531-579 bp	1.0	81.8
trnH-GTG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	533-581 bp	1.0	81.8
trnH-GTG-psbA_p5	GATCCACTTGGCTACATCCG	ATCGTGCTAACCTTGGGATG	55.0	50.0	58.1	57.7	568-616 bp	1.0	79.6