Hotspot

ycf1

SSC · 108660-114026 · 5367 bp

Back to Glycine

Pi 0.0215

MarkerSeek score 52.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5367
variable_sites: 327
pi: 0.0215
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGGCTTGTGGAAGAAGGGA	TTGGATTTGTCCACGAAAGC	50.0	45.0	58.0	56.9	1234-1243 bp	0.818182	67.9
ycf1_p2	CCTTCTTCGAGCTAGGCTTG	TTGGATTTGTCCACGAAAGC	55.0	45.0	58.1	56.9	1246-1255 bp	0.818182	67.7
ycf1_p3	TAGGCTTGTGGAAGAAGGGA	TGGATTTGTCCACGAAAGCG	50.0	50.0	58.0	59.1	1233-1242 bp	0.818182	67.5
ycf1_p4	AGGGCCCTCTTATCTCTTCC	TTGGATTTGTCCACGAAAGC	55.0	45.0	57.9	56.9	1264-1273 bp	0.818182	67.3
ycf1_p5	GGGCCCTCTTATCTCTTCCT	TTGGATTTGTCCACGAAAGC	55.0	45.0	57.9	56.9	1263-1272 bp	0.818182	67.3