Hotspot

accD

LSC · 57408-58934 · 1527 bp

Back to Glycine

Pi 0.026

MarkerSeek score 63.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 102
pi: 0.026
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TATTTCCTTGAACGAGGCGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.6	58.0	2234-2554 bp	1.0	84.0
accD_p2	TATTTCCTTGAACGAGGCGC	GTGGCCCCTTGTGGAATAAA	50.0	50.0	58.6	58.1	2579-2899 bp	1.0	83.5
accD_p3	TATTTCCTTGAACGAGGCGC	TGCAACCAACAATAACCCCA	50.0	45.0	58.6	57.9	2472-2792 bp	1.0	83.3
accD_p4	TATTTCCTTGAACGAGGCGC	TTTCCAGGAAATCCCCAACG	50.0	50.0	58.6	58.1	2720-2991 bp	0.818182	75.6
accD_p5	TATTTCCTTGAACGAGGCGC	CAGGAAATCCCCAACGAACA	50.0	50.0	58.6	58.1	2716-2944 bp	0.727273	70.0