Hotspot

trnL-UAG-rpl32

SSC · 123865-124149 · 285 bp

Back to Glycine

Pi 0.0301

MarkerSeek score 71.20

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 285
variable_sites: 25
pi: 0.0301
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	GCGCAATCTATTTTTACGGGG	50.0	47.6	57.9	58.0	349-396 bp	1.0	80.6
trnL-UAG-rpl32_p2	AGCAGCGTGTCTACCAATTT	GCGCAATCTATTTTTACGGGG	45.0	47.6	57.8	58.0	342-389 bp	1.0	80.2
trnL-UAG-rpl32_p3	CCTAAGAGCAGCGTGTCTAC	GCGCAATCTATTTTTACGGGG	55.0	47.6	57.8	58.0	348-395 bp	1.0	80.1
trnL-UAG-rpl32_p4	CTAAGAGCAGCGTGTCTACC	GCGCAATCTATTTTTACGGGG	55.0	47.6	57.8	58.0	347-394 bp	1.0	80.1
trnL-UAG-rpl32_p5	AAGATGTCATGCCGCTACTC	GCGCAATCTATTTTTACGGGG	50.0	47.6	57.8	58.0	399-446 bp	1.0	79.3