Hotspot

psaC-ndhD

SSC · 120833-120967 · 135 bp

Back to Glycine

Pi 0.0334

MarkerSeek score 66.20

Primer available yes

Label psaC-ndhD

Feature Metadata

label_name: psaC-ndhD
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 14
pi: 0.0334
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhD_p1	AAACAACTCGAAGCATGGGT	CCCCCGACTATTGGCAAAAT	45.0	50.0	58.0	58.2	213-220 bp	1.0	87.0
psaC-ndhD_p2	GGCATGAAACAACTCGAAGC	CCCCCGACTATTGGCAAAAT	50.0	50.0	58.0	58.2	219-226 bp	1.0	86.6
psaC-ndhD_p3	AACAACTCGAAGCATGGGTC	CCCCCGACTATTGGCAAAAT	50.0	50.0	58.5	58.2	212-219 bp	1.0	83.3
psaC-ndhD_p4	GAAACAACTCGAAGCATGGG	CCCCCGACTATTGGCAAAAT	50.0	50.0	57.4	58.2	214-221 bp	1.0	82.1
psaC-ndhD_p5	CAACTCGAAGCATGGGTCTA	CCCCCGACTATTGGCAAAAT	50.0	50.0	57.3	58.2	210-217 bp	1.0	81.9