Hotspot

ycf1-rps15

SSC · 114027-114442 · 416 bp

Back to Glycine

Pi 0.0274

MarkerSeek score 58.00

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 416
variable_sites: 34
pi: 0.0274
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GAACCAGTTCGCCTGTCTAG	ATGCATTGATGGGTGGTGAG	55.0	50.0	58.0	58.2	1385-1411 bp	1.0	82.9
ycf1-rps15_p2	GAACCAGTTCGCCTGTCTAG	CCCAACTGTTCTGGTCCATT	55.0	50.0	58.0	57.7	1585-1611 bp	1.0	82.0
ycf1-rps15_p3	GAACCAGTTCGCCTGTCTAG	TTTCCCAACTGTTCTGGTCC	55.0	50.0	58.0	57.6	1588-1614 bp	1.0	81.5
ycf1-rps15_p4	GAACCAGTTCGCCTGTCTAG	CATGCATTGATGGGTGGTGA	55.0	50.0	58.0	58.5	1386-1412 bp	1.0	80.5
ycf1-rps15_p5	ATCCGCGAGTGAAAATTCGT	ATGCATTGATGGGTGGTGAG	45.0	50.0	58.3	58.2	880-900 bp	0.818182	71.6