Hotspot

rps3-rps19

LSC · 83011-83361 · 351 bp

Back to Glycine

Pi 0.0358

MarkerSeek score 67.50

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 42
pi: 0.0358
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TGATCCATTCAACACGTGCA	ATTTCCGAGGACATGCGAAA	45.0	45.0	58.1	57.9	932-964 bp	1.0	84.6
rps3-rps19_p2	TGATCCATTCAACACGTGCA	TCCGAGGACATGCGAAAAAT	45.0	45.0	58.1	57.9	929-961 bp	1.0	84.6
rps3-rps19_p3	TGATCCATTCAACACGTGCA	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.1	58.1	1340-1412 bp	1.0	83.3
rps3-rps19_p4	TGATCCATTCAACACGTGCA	GTCGGACAAGTGGGAAATGT	45.0	50.0	58.1	58.1	1479-1551 bp	1.0	82.5
rps3-rps19_p5	TGATCCATTCAACACGTGCA	ATTACCTTCTGGGGAGGTCC	45.0	55.0	58.1	58.1	1528-1600 bp	1.0	82.4